透析机的工作原理主要是通过流过透析器的水的压力(正压或负压)透出病人血液中的有害物质或析人补充有益液体,达到维持病人体内介质平衡,起到治疗作用.只要了解机器的结构和工作原理,按照要求正确操作使用机器,经常维护清洗有关部件,及...透析机的工作原理主要是通过流过透析器的水的压力(正压或负压)透出病人血液中的有害物质或析人补充有益液体,达到维持病人体内介质平衡,起到治疗作用.只要了解机器的结构和工作原理,按照要求正确操作使用机器,经常维护清洗有关部件,及时处理机器异常故障现象,就能减少机器的故障发生率提高机器的利用率,对病人的透析安全提供保障.下面是我院在使用Bellc Mul TMAT plus system血液透析机过程中出现的两例故障及解决办法.展开更多
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for many years to rapidly identify whole bacteria. However, no consistent methodology exists for the rapid iden...Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for many years to rapidly identify whole bacteria. However, no consistent methodology exists for the rapid identification of bacteria cultured in liquid media. Thus, in this study we explored the use of MALDI-TOF MS analysis for rapid identification of cells cultured in liquid media. We determined that 2,5-dihydroxybenzoic acid (50 mg mL-1, 50% acetonitrile, 0.1% trifluoroacetic acid) was the best matrix solution for MALDI-TOF MS for this type of study. Moreover, the tested strains were successfully differentiated by principal component analysis, and the main characteristics of the mass peaks for each species were found in mixed culture samples. In addition, we found that the minimum number of cells for detection was 1.8×103. In conclusion, our findings suggest that MS-based techniques can be developed as an auxiliary method for rapidly and accurately identifying bacteria cultured in liquid media.展开更多
文摘透析机的工作原理主要是通过流过透析器的水的压力(正压或负压)透出病人血液中的有害物质或析人补充有益液体,达到维持病人体内介质平衡,起到治疗作用.只要了解机器的结构和工作原理,按照要求正确操作使用机器,经常维护清洗有关部件,及时处理机器异常故障现象,就能减少机器的故障发生率提高机器的利用率,对病人的透析安全提供保障.下面是我院在使用Bellc Mul TMAT plus system血液透析机过程中出现的两例故障及解决办法.
基金supported by the National Science and Technology Major Project (Grant No. 2008ZX10207) the Innovation Method Fund of Ministry of Science and Technology of China (Grant Nos. 2008IM022000 and 2009IM030300) the National Science and Technology Support Program (Grant No. 2008BAK41B0)
文摘Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for many years to rapidly identify whole bacteria. However, no consistent methodology exists for the rapid identification of bacteria cultured in liquid media. Thus, in this study we explored the use of MALDI-TOF MS analysis for rapid identification of cells cultured in liquid media. We determined that 2,5-dihydroxybenzoic acid (50 mg mL-1, 50% acetonitrile, 0.1% trifluoroacetic acid) was the best matrix solution for MALDI-TOF MS for this type of study. Moreover, the tested strains were successfully differentiated by principal component analysis, and the main characteristics of the mass peaks for each species were found in mixed culture samples. In addition, we found that the minimum number of cells for detection was 1.8×103. In conclusion, our findings suggest that MS-based techniques can be developed as an auxiliary method for rapidly and accurately identifying bacteria cultured in liquid media.